Circulating Microvesicles Association with Oxidative and Chronic Stress in Healthy and Coronary Artery Disease Affected Male Populations

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Laboratorinė medicina. 2019,
t. 21,
Nr. 2,
p. 51 -

Introduction. Endothelial microvesicles (EMV) could be potential biomarkers for early diagnosis of myocardial infarction yet their release in post myocardial infarction and association with oxidative stress largely not studied. The objective was to determine the differences in EMV numbers in patients after myocardial infarction and their association with oxidative stress in comparison with healthy controls.

Materials and Methods. 15 post myocardial infarction (MI) patients and 81 healthy controls were included into the study. Blood plasma samples tested using BD Fortessa cytometer (BD, San Jose). Three EMV popuiations were examined: 1) CD144+, CD42a-, CD61-, 2) CD144+, CD42a+, CD61-, 3) CD105+, CD42a-, CD61- and percentage of EMV, expressing CD62e was determined. Malondialdehyde (MDA) concentration determined by a Nexera X2 UHPLC system (Shimadzu, Japan).

Results. Individuals participating in the study grouped into three groups: 1) younger healthy group (25-39 year old), 2) elder healthy group (40-60 year old) and 3) patients (40-60 year old). CD62e expression on EMV membrane was higher in patients group in comparison to control younger and elder groups. MDA concentration median was higher in patients group. EMV populations and their activation levels were associated with MDA concentration only in the control groups at different age intervals.

Conclusions. Activation of EMV was higher in post MI group in all populations of endothelial microvesicles except for CD144+ and CD42a-. Counts of ciriuiating EMV popui aiions and their CD62e expression showed associations with oxidative and chronic stress markers.


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