Primary Hemostasis Biomarkers and Possibilities of Assays
Aušra Mongirdienė
Summary
These days devices and their systems to analyze homeostasis are working in different ways and do not have similar characteristics. Unfortunately, because of significant variety of platelet defects none of the devices provide 100 per cent accuracy. In order to avoid mistakes, several types of analysis have to be used. It is attempted in this article to review the methods to analyze initial homeostasis that are used the most often and ones that were used eart ier. Also this article tries to distinguish their potential, advantages and disadvantages, but before that briefly describes platelets morphology and physiology of initial homeostasis. The most of the attention is paid to platetets’ structures that are important while judging their function: plasmid membrane, that has the receptors that are important for interaction of platelets with other cells, compounds and granules that includes compounds like p tromboglobulin and 4th platelets factor can be studted in order to evaluate quality of secretion from platelets. Primary homeostasis laboratory read-outs like P-selektin, p tromboglobulin, 4th platelets factor, trombocsan B2 and platelets’ receptors are also discussed. Processes, that takes place during the primary homeostasis, because of the attendance of particular platelets’ receptors: adhesion, activation and aggregation. The means of testing of primary homeostasis: blood swab, exploration of number of platelets, methods of bleeding time test, methods of aggregometry (turbi- dimetric and whole blood), luminometric test of release reactions, determinations of secretions’ read-outs, cytometry of flow. The most economy and reliable test of platelets functions - turbidimetric test by Born is described more extensively.
Keywords: platelet function, bleeding time, aggregation, PFA-100, flow cytometry.
