Summary
Introduction. High-density lipoproteins have antiatherogenic, antioxidant properties which help to attenuate the development of atherosclerosis. Hydroperoxides are mainly associated with HDL particles in serum. Currently, it is hypothesized that HDLs may accumulate hydroperoxide molecules by absorbing them from other lipoproteins. Because of this removal of excess hydroperoxide molecules other lipoproteins are protected from the accumulation of lipid peroxidation products which may prevent further oxidation and progression of atherosclerosis.
Materials and methods. 43 healthy men were included into the study. The anthropometric measurements of waist circumference, BMI and blood serum lipid (total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides), inflammation (hs-CRP), lipid oxidation (hydroperoxides, MDA) and antioxidant (carotenoid) markers were evaluated. Carotenoid and MDA concentrations were evaluated by liquid chromatography and hydroperoxides were measured using modified d-ROM test – Fe-ROM.
Results. The majority (75.89%) of serum hydroperoxides are associated with the HDL fraction. There was a strong positive correlation between serum hydroperoxide and HDL fraction hydroper oxide concentrations (r=0.651, p=0.001). There were statistically significant differences between serum concentrations of total cholesterol and lycopene medians among different HDL fraction hydroper oxide concentration groups (p<0.05). There were no statistically significant differences found between age, waist circumference, BMI, serum concentrations of HDL-cholesterol, LDL-cholesterol, triglycerides, malondialdehyde and carotenoids among different HDL fraction hydroperoxide concentration groups (p>0.05).
Conclusions. High-density lipoproteins are associated with the majority of serum hydroperoxides. There were statistically significant differences between serum concentration of total cholesterol and carotenoid lycopene medians among different hydroperoxide concentration groups.
