Background. Adult mesenchymal stem cells (MSC) have generated a great deal of interest for their potential use in re - generative medicine. In further research of the MSC role in tissue regeneration and their possible uses for therapeutical reasons it is important to understand, how mediators of inflammation and other products produced by activated neutrophils act on MSC migration. The goal of this research was to estimate the intlu - ence of the substances produced and released by activated neutrophils on the migratory activity of MSC.
Methods. Migratory activity of MSC was investigated using a 10-well chemotaxis chamber with polycarbonate filters with 8 ^m pores. Evaluation of completed transmigration was performed on the stained filters under a light microscope at 20* magnification. The number of migrated cells in control and stimulated wells was counted for random fields per well and the average of the transmigrated cells in each well was calculated. Neutrophils isolated from the blood of healthy volunteers were activated in two different manners, by fMLP and by PMA.
Results. An average number ofMSC transmigrated towards the supernatant of activated neutrophils was 26.28 ± 1.25 vs. 12.12 ± 1.98 in control group. An average number of MSC suspended in the supernatant of fMLP activated neutrophils and transmigrated towards DMEM suplemented with 1% human plasma was ± 2.00 vs. 27.96 ± 2.36 in control group. Similarly, an average number of MSC suspended in the supernatant of PMA activated neutrophils and migrated towards DMEM suplemented with 1% human plasma was 12.84 ± 1.01 vs. ± 1.62 in control group.
Conclusions. The supernatant of activated neutrophils was an effective inducer for chemotaxis of MSC. The products of neutrophils degranulation released after their activation by fMLP partly arrested MSC. Similarly, the products of neutrophils degranulation and reactive oxygen species released after activation by PMA also caused statistically significant reduction in MSC chemo- kinetic activity.
