Summary
Background. To estimate coagulase-negative staphylococci prevalence in community and to assess their level of resistance to antibiotics.
Material and methods. In 2013-2014, 204 district area popul ation and 53 undergraduates were investigated. The test material from the nostrils was taken to Amies transport medium. The test material was sown on mannitol salt agar. Staphylococci cultures were identified after plasmacoagulase, DNA-ase, latex agglutination, oxidase and furazolidone tests. Coagulase-negative staphylococci strains were identified to species Microgen Staph ID test and found them acting antibiotic MIC by GPN3F Sensititre plates. Resistance to antibiotics study was assessed under the NCCLS and EUCAST recommendations.
Results. 242 isolates were studied of coagulase-negative staphylococci: 193 (94.6%) of the population and 49 (92.4%) of the students. 105 coaguliase-negative staphylococci strains resistant to antibiotics were identified. Most prevalent coagulase-negative staphylococci in human nostrils are Staphylococcus hominis subsp. hominis (25.7%), Staphylococcus warneri (12.4%), Staphylococcus haemolyticus (8.5%) and Staphylococcus chromogenes (7.6%). 60.6% of methicillin-susceptible strains of Coagulase-negative staphylococci are resistant to penicillin, 57.5% - to erythromycin, 36.2% - to ampicillin, 20.2% - to tetracycline. 11 Coagulase-negative staphylococci strains are resistant to methicillin, among them 8 (72.7%) are resistant to tetracycline, 5 (45.4%) to erythromycin and 4 (36.3%) to clindamycin, all strains were susceptable to vancomycin.
Conclusions. Coagulase-negative staphylococci with the ability to acquire resistance to antibiotics and spread among humans constitute a real threat to human health. In community have been dominated by Staphylococcus hominis subsp. hominis and Staphylococcus warneri. 10.4% isolated from community coagulase-negative staphylococci strains were resistant to methicillin.
Keywords: coagulase-negative staphylococci, prevalence, antibiotic resistance, methicillin resistance.
