The Impact of Narrowband Ultraviolet A1 on Active Caspase-3 Expression in Scleroderma Animal Model

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Laboratorinė medicina. 2017,
t. 19,
Nr. 3,
p. 151 -
157

Background. To define the impact of 365±5 nm ultraviolet A1 (UVA1) radiation for the dermal fibrosis and apoptosis in bleomycin (BLM)-induced mouse model of scleroderma.

Materials and methods. Forty two DBA/2 strain healthy mice and mice with bleomycin-induced scleroderma were treated with high and medium doses of UVA1. The average cumulative doses were 1200 J/cm2 for high and 600 J/cm2 for medium dose treatments. Nontreated groups served as the control. Light source emitting a narrowband UVA1 365±5 nm and radiation of 21 mW/cm2 power density was used in the study. Histological analysis with H&E staining for dermal thickness measurement and immunohistochemical staining for active caspase-3 proteins were performed. Statistical significance was expressed by a P value <0.05.

Results. The dermal thickness of mice treated with high and medium doses of UVA1 was significantly lower in compari - son to the control BLM group (P<0.05). After irradiation with cumulative doses of 1200 J/cm2 and 600 J/cm2 of UVA1 on mice with scleroderma, the expression of active caspase-3 was significantly (P<0.05) higher (17.2±3.3% and 17.1±4.4%, respectively) as compared with that in the BLM control group (9.8±1.5%) The expression profile of aci tive caspase-3 in the skin did not differ between healthy and UVA1-treated healthy mice groups.

Conclusions. Narrowband UVA1 phototherapy effectively reduced the dermal thickness, and the impact was dose-dependent. UVA1 radiation caused the double increase of the active caspase-3 expression in the skin of mice with scleroderma.

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