Microsatellite alterations in bladder cancer

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Microsatellite alterations in bladder cancer

Diana Stančiūtė1, Agnė Barzinskie, Genovefa Chvatovič1, Inga Baranauskaitė2, Giedrė Smailytė1, Albertas Ulys1, Janina Didžiapetrienė1' 2

 

Background. Microsatellite alterations are important in understanding the mechanisms of bladder cancer carcino­genesis as well as in the diagnostic and prognostic aspects. There are already carried out a multicentre studi es to de­termine a diagnostic value of the micro­satellites alterations, but it is important to evaluate the predictive value of these alterations, research is limited and data are only starting to accumulate.

Aim of the study was to determine the frequency of microsatellite alter­ations in superficial bladder tumors and to estimate their importance for disease free period.

Material and methods. This study included 25 patients with bladder cancer, surgically treated at Department of Onco- urology at Institute of Oncology, Vilnius University. Five microsatellite markers, CYAR04, D5S818, D13S317, I-FABP and TH01, were identified in the tumor tissue and blood samples. DNA was isolated from all samples. Microsatellite sequences were amplified by polymerase chain reaction (PCR) and products were separated on vertical and capillary electrophoresis.

Results. The microsatellite alter­ations were determined in 48 percent of analyzed tumor tissue samples. An anal­ysis of five genome regions showed the al­terations mostly for CYAR04 microsatel­lite marker. The correlation between microsatellite alterations and disease free period was not identified.

Conclusions. Further research should be focused on the question are these alterations related to microsatellite instability or to the major parts of the ge­nome damage, or microsatellite instabil­ity could serve to interpret the response to treatment and evaluat ion of disease progno sis.

Keywords: microsatellite alterations, bladder cancer, disease free period.

 

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