Impact of growth factors on miogenic mouse cells proliferation and surface markers expression

Impact of growth factors on miogenic mouse cells proliferation and surface markers expression

Radvile Malickaitė^1'2, Laimutė Jurgauskienė¹' ², Jolanta Bukauskaitė³

Background. Acute myocardial infarc­tion is accompanied by increased levels of proinflammatory cytokines, including TNF-a. We aimed to analyze alternations in myogenic mouse stem cells profifera - tion as well as the surface expression of adhesion molecules and receptors, in­volved in cell differentiation and trans­endothelial migration.

Material and methods. Skeletal muscle derived myogenic cell lines were isolated from 2-4 week old mouse leg musculus. Cell proliferation was as­sessed by flow cytometry using BrdU Flow Kit (BD Biosciences, San Diego). In­fluence of cytokines of pharmacological potential TNF-a, G-CSF, SCF and G-CSF/SCF combination on myogenic mouse stem cells proliferation as well as surface molecules expression has been investigated.

Results. High (100 ng/ml) concentra­tions of TNF-a inhibited cells prolifera­tion; no stimulation of cell cycle has been noticed while using growth factors (G-CSF, SCF and G-CSF/SCF combina - tion). Endothelial progenitor cell mark­ers CD31 and CD146 were not expressed. Neither TNF-a, no G-CSF, SCF nor G-CSF/SCF combination has influenced the expression of adhesion molecule CD44. Expression of CD81 (TAPA-1) and CD9 were inhibited using TNF-a, and in­duced only by G-CSF/SCF combination. SCF ligand CD117 expression was found to be twice as high after the incubation with growth factors combination in com­parison with controls.

Conclusions. It was shown, that high concentrations of proinflammatory cytokine TNF-a suppress myogenic stem cell proliferation, as well as adhesion re­ceptors CD81 (TAPA-1) and CD9 expres­sion. No significant effect of SCF (100 ng/ml) or G-CSF (100 ng/ml) on cell pro lif er a tion was es tab lished. The G-CSF/SCF combination (100 ng/ml each) activates trans-membrane protein with tyrosine kinase capacity SCF ligand CD117 expression on myoblast cell line.

Keywords: stem cell, cytokines, adhe­sion molecules.